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Index > Protein center > MB(Gene name) > Human
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  • MB (Gene name),
  • Myoglobin (Protein name ),  MYG_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Gene name:
    MB;
    Protein name:
    Myoglobin;
    Alternative:

    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
    Subcellular Location:
    N/A
    Protein Attributes:
    Sequence length:
    154
    Sequence:
    50:
    MGLSDGEWQL | VLNVWGKVEA | DIPGHGQEVL | IRLFKGHPET | LEKFDKFKHL | 
    100:
    KSEDEMKASE | DLKKHGATVL | TALGGILKKK | GHHEAEIKPL | AQSHATKHKI | 
    150:
    PVKYLEFISE | CIIQVLQSKH | PGDFGADAQG | AMNKALELFR | KDMASNYKEL | 
    154:
    GFQG
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    KEGG:
    SMR:
    MIM:
    UniGene:
    String:
    Pfam:
    Uniprot:
     
    FOR
    ELISA Kit for Human Myoglobin
    MSDS:
    Please sign in first.
    ELISA Kit for Human Myoglobin
    Cat.:
    E0480h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Myoglobin
    Cat.:
    E0480r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Myoglobin
    Cat.:
    E0480d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Human Myoglobin
    Cat.:
    E0480m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Myoglobin
    Cat.:
    E0480p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Myoglobin
    Cat.:
    E0480c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Human Myoglobin
    Cat.:
    E0480b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Myoglobin
    Cat.:
    U0480b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Myoglobin
    Cat.:
    U0480p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Myoglobin
    Cat.:
    U0480r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Myoglobin
    MSDS:
    Please sign in first.
    CLIA Kit for Human Myoglobin
    Cat.:
    U0480h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Myoglobin
    Cat.:
    U0480m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Myoglobin
    Cat.:
    U0480c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Myoglobin
    Cat.:
    U0480d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human Myoglobin
    Polyclonal Antibody for Human Myoglobin
    Polyclonal Antibody for Human Myoglobin
    Polyclonal Antibody for Human Myoglobin
    Cat.:
    P0480Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Myoglobin
    Cat.:
    P0480Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Myoglobin
    Polyclonal Antibody for Human Myoglobin
    Polyclonal Antibody for Human Myoglobin
    Cat.:
    P0480Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human Myoglobin
    Monoclonal Antibody for Human Myoglobin
    Monoclonal Antibody for Human Myoglobin
    Monoclonal Antibody for Human Myoglobin
    Monoclonal Antibody for Human Myoglobin
    Cat.:
    M0480h
    Price:
    Please sign in first.
    Packing:
    40ug
    Monoclonal Antibody for Human Myoglobin
    Monoclonal Antibody for Human Myoglobin
    Monoclonal Antibody for Human Myoglobin
    Protein for Human Myoglobin
    Protein for Human Myoglobin
    Protein for Human Myoglobin
    Protein for Human Myoglobin
    Protein for Human Myoglobin
    Protein for Human Myoglobin
    Protein for Human Myoglobin
    Protein for Human Myoglobin

    R&D Technical Data
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    s

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    s

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    References
    1. 1.
      "Organization of the human myoglobin gene."
      Weller P. , Jeffreys A.J. , Wilson V. , Blanchetot A.
      EMBO J.3:439-446(1984) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    2. 2.
      "Cloning of the human myoglobin gene."
      Akaboshi E.
      Gene33:241-249(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    3. 3.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
    4. 4.
      "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L. , Schick M. , Neubert P. , Schatten R. , Henze S. , Korn B.
      Submitted (2004-06) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
    5. 5.
      NIEHS SNPs program
      Submitted (2005-04) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    6. 6.
      "The DNA sequence of human chromosome 22."
      Dunham I. , Hunt A.R. , Collins J.E. , Bruskiewich R. , Beare D.M. , Clamp M. , Smink L.J. , Ainscough R. , Almeida J.P. , Babbage A.K. , Bagguley C. , Bailey J. , Barlow K.F. , Bates K.N. , Beasley O.P. , Bird C.P. , Blakey S.E. , Bridgeman A.M. , more...
      Nature402:489-495(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    7. 7.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Skeletal muscle.
    8. 8.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-154
    9. 9.
      "The myoglobin of primates. I. Hylobates agilis (gibbon)."
      Romero-Herrera A.E. , Lehmann H.
      Biochim. Biophys. Acta251:482-488(1971) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SEQUENCE REVISION TO 20-23 AND 84
    10. 10.
      "The myoglobin of primates. II. Pan troglodytes (chimpanzee)."
      Romero-Herrera A.E. , Lehmann H.
      Biochim. Biophys. Acta278:62-67(1972) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SEQUENCE REVISION TO 100-102
    11. 11.
      "The human myocardial two-dimensional gel protein database: update 1994."
      Corbett J.M. , Wheeler C.H. , Baker C.S. , Yacoub M.H. , Dunn M.J.
      Electrophoresis15:1459-1465(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-21
      tissue: Heart.
    12. 12.
      "Abnormal human myoglobin: 53 (D4) glutamic acid-->lysine."
      Boulton F.E. , Huntsman R.G. , Lorkin P.A. , Lehmann H.
      Nature223:832-833(1969) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT LYS-55
    13. 13.
      "The third variant of human myoglobin showing an unusual amino acid substitution: 138(H16)arginine-->tryptophan."
      Boulton F.E. , Huntsman R.G. , Romero Herrera A. , Lorkin P.A. , Lehmann H.
      Biochim. Biophys. Acta229:716-719(1971) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT TRP-140
    14. 14.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT ASN-134
    15. 15.
      "The second variant of human myoglobin; 138(H16) arginine leads to glutamine."
      Boulton F.E. , Huntsman R.G. , Yawson G.I. , Romero-Herrera A.E. , Lorkin P.A.
      Br. J. Haematol.20:69-74(1971) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT GLN-140
    16. 16.
      "X-ray crystal structure of a recombinant human myoglobin mutant at 2.8-A resolution."
      Hubbard S.R. , Hendrickson W.A. , Lambright D.G. , Boxer S.G.
      J. Mol. Biol.213:215-218(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF MUTANT ARG-46 AND ALA-111 IN COMPLEX WITH HEME
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